
MYKOS
The Sealants Team
Overview
Project Summary & Research Focus
This team is working to improve the water resistance of mycelium-based composites using natural, plant-based sealants. The project involves testing two specific coatings: an oil-wax blend featuring a hydrophobic
nanostructure formed via salt templating, and a sealant derived from agave wax. Both will be tested on substrates with different compression levels using ASTM protocols to measure water resistance and durability.
Our Goals
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Improve the water resistance of mycelium-based composites using natural, plant-based sealants.
01
Compare and identify different natural materials and processes suitable for water resistance.
02
Apply standardized testing
protocols (ASTM) to assess and compare the effectiveness of developed sealants.
03


Agave Wax Samples
When we extracted the wax from our agave leaf samples, it only generated a small amount. This taught us that scaling this process would be challenging.


Salt Nanocrystal Oil-Wax Solutions
This method performs better than the agave wax method as a waterproofing agent.


Water Testing
We tested each composite, with and without sealants, for water drop angle and time to absorption.
Our Discoveries
On wood substrates, all oil-wax sealants produced a water contact angle of greater than 90 degrees, but less than 150 degrees, classifying it as hydrophobic. Agave-based sealants all produced a water contact angle of less than 90 degrees, classifying it as hydrophilic.
On wood substrates, all oil-wax sealants reduced the water contact angle loss over 150 seconds. Agave-based sealants from an old agave leaf (used 30 days after pruning) reduced water contact angle loss over 150 seconds, while agave-based sealants from a new agave leaf (used 1 day after pruning) increased water contact angle loss over 150 seconds.
Mycelium-based composites without an oil-wax sealant completely absorbed a 25-microliter water drop in less than 30 seconds. When coated, the complete absorption of a 25-microliter water drop is over 180 seconds, suggesting that the oil-wax sealant decreases the porosity of mycelium-based composites.
The complete water absorption of mycelium-based composites for 24 hours with and without an oil-wax sealant is highly varied. When the oil-wax sealant was applied, some species-substrates types absorbed less water and others absorbed more water compared to the unsealed samples.
Meet Our Research Team

Dharyl Licudine
3rd year, Molecular and Medical Microbiology
Oversees experimental plans, procedures, and reports

Phuong Tran
3rd year, Genetics & Genomics
Help with experiments, documentation, and reports

Gisella Henrianto
1st year, Biological Science
Help with experiments, documentation, reports

Keiko Ladrillono
2nd year, Wildlife, Fish, and Conservation Biology
Social media lead, videographer, help with experiments and planning procedures













